Portnoy then said, 'I'm gonna f***ing make it my life f***ing mission to ruin these people. Like, I'm coming for your throat. I'm never ending.'
He explained that he'd spent the hours prior to making the video tracking down as much information as possible on the situation.
'You think I'm gonna put up with this s**t at my bar?,' Portnoy explained.
The 48-year-old Portnoy said he was 'hunting down' the waitresses that served the table and those at the table themselves, 'Because I want f***ing consequences for f***ing actions.'
Portnoy said that both of the waitresses responsible for holding up the sign have been fired and called one of them 'one of the dumbest f***ing humans I've ever talked to in my life.'
Video of the sign was posted onto an Instagram story of an account that appears to have been deleted.
That story tagged a now-private account. Portnoy says he spoke to the person behind the tagged account and revealed that they weren't at the bar that evening.
'I had this kid basically in tears, turning state witness to me, rolling on the people who there,' he said.
Portnoy says he contacted the person who posted the video before the account was deleted. The Barstool founder claims is a student at Temple University. DailyMail.com has reached out to the school for comment.
He says the person behind the video began crying and telling Portnoy that he isn't anti-Semitic.
'I don't buy it for a second,' Portnoy said. 'Who the f*** would do that sign in my f***in bar? You think I'm just gonna sit here? I'm gonna make it my mission to put you in lights.'
He continued: 'Tell me how do I make this right before I go ape-s**t. You want to take anti-Semitism classes? You want to do whatever? You make it feel right. I don't need your tears... Trust me, they're pulling the footage.
'I'm 20 steps ahead and I'm using everything. I am going to come for your f***ing throat unless you give me answers.'