Harvard researchers have successfully reversed aging in mice using a drug cocktail which you can purchase right now

Harvard researchers have successfully reversed aging in mice using a drug cocktail which you can purchase right now

A hallmark of eukaryotic aging is a loss of epigenetic information, a process that can be reversed. We have previously shown that the ectopic induction of the Yamanaka factors OCT4, SOX2, and KLF4 (OSK) in mammals can restore youthful DNA methylation patterns, transcript profiles, and tissue function, without erasing cellular identity, a process that requires active DNA demethylation. To screen for molecules that reverse cellular aging and rejuvenate human cells without altering the genome, we developed high-throughput cell-based assays that distinguish young from old and senescent cells, including transcription-based aging clocks and a real-time nucleocytoplasmic compartmentalization (NCC) assay. >We identify six chemical cocktails, which, in less than a week and without compromising cellular identity, restore a youthful genome-wide transcript profile and reverse transcriptomic age. Thus, rejuvenation by age reversal can be achieved, not only by genetic, but also chemical means.

aging-us.com/article/204896/text

In 2006, Takahashi and Yamanaka demonstrated that the expression of four transcription factors, OCT4, SOX2, KLF4, and c-MYC (collectively known as “OSKM”), reprograms the developmental potential of adult cells, enabling them to be converted into various cell types [17, 18]. These findings initiated the field of cell reprogramming, with a string of publications in the 2000s showing that the identity of many different types of adult cells from different species could be erased to become induced pluripotent stem cells, commonly known as “iPSCs” [17, 19–21].

The ability of the Yamanaka factors to erase cellular identity raised a key question: is it possible to reverse cellular aging in vivo without causing uncontrolled cell growth and tumorigenesis? Initially, it didn’t seem so, as mice died within two days of expressing OSKM. But work by the Belmonte lab, our lab, and others have confirmed that it is possible to safely improve the function of tissues in vivo by pulsing OSKM expression [22, 23] or by continuously expressing only OSK, leaving out the oncogene c-MYC [7, 8]. In the optic nerve, for example, expression of a three Yamanaka factor combination safely resets DNA methylomes and gene expression patterns, improving vision in old and glaucomatous mice via a largely obscure mechanism that requires TET DNA demethylases [8]. Numerous tissues, including brain tissue, kidney, and muscle, have now been reprogrammed without causing cancer [7, 8, 22, 24, 25]. >In fact, expression of OSK throughout the entire body of mice extends their lifespan [26]. Together, these results are consistent with the existence of a “back-up copy” of a youthful epigenome, one that can be reset via partial reprogramming to regain tissue function, without erasing cellular identity or causing tumorigenesis [7–9].

To achieve age reduction without altering cell identity, we focused on small molecules that were likely to work in the early stages of CiPSC formation, including valproic acid (V), CHIR-99021 (C), E-616452 (6), tranylcypromine (T) and forskolin (F). Previous studies of reprogramming efficiency with small molecules demonstrated that either OCT4 alone or SKM, when combined with VC6T or F, respectively, can generate iPSCs, and VC6TF facilitates a mesenchymal-to-epithelial transition, an early stage of reprogramming in mouse cells [31, 53]. Because of the known differences in differentiation between mice and humans, we also investigated molecules that have been reported for the initiation states of generating human CiPSCs including CHIR-99021 (C), E-616452 (6), TTNPB (N), Y-27632 (Y), Smoothened Agonist (S), and ABT-869 (A) [30]. The molecules VC6TF (Cocktail 1: C1) and C6NYSA (Cocktail 4: C4) were used as basal reprogramming cocktails and supplemented with other boosters known to increase iPSC efficiency, including sodium butyrate, basic fibroblast growth factor (bFGF), and alpha ketoglutarate (α-KG) (Figure 3A, 3B, Supplementary Tables 1 and 2) [54].

Can I just eat the mouse?

Based on the fact that iPSCs can also be generated using SKM or O alone [55, 56], we evaluated the effect of the boosters on VC6T (SKM alternative) and F (O alternative). We also assessed the effect of combinations including C6N, because it has been reported that the removal of Y, S, or A from Cocktail 4 (C6NYSA) did not reduce the CiPSC efficiency [30]. Among 80 cocktails tested in the NCC assay, the VC6TF basal cocktail was the most effective at restoring the integrity of nucleocytoplasmic compartmentalization, a key sign of age-reversal (Figure 3B). A recent, unpublished study reported that 6T pre-treatment prevents senescence in human fibroblasts, and 6, T, or 6T extends the lifespan of Caenorhabditis elegans by up to 42.1% [57]. We, however, saw no benefit of F alone or the VC6T cocktail on reversing senescence phenotypes in our system (Figure 3B). Next, we chose six cocktails of small molecules for further investigation, three of which were based on Cocktail 1 as well as two additives (referred to as Cocktail 2 and 3) and the other three based on Cocktail 4 plus additional additives (referred to as Cocktail 5 and 6) (Supplementary Table 2). Sodium butyrate, a histone deacetylase inhibitor, was one of the most effective additives in both human and mouse cocktails (C2 and C5). Basic fibroblast growth factor (bFGF) was used for Cocktail 3, while α-KG was included in Cocktail 6. To better gauge the effect of these compounds on NCC integrity, we used Pearson’s correlation to assess the distribution of fluorescent proteins (Figure 3C, 3D). The six cocktails statistically improved compartmentalization in senescent cells, both in terms of correlation analysis (Figure 3C) and imaging of NCC signaling (Figure 3D).

All the ingredients in cocktail 2 can be bought online

Look up the food, drug, and alcohol interactions for each of these so you lower the chances of things going wrong, I know with tranylcypromine you can't eat high tyramine things while on it (tyramine is found in aged and fermented foods)

-Valproic acid:
aipctshop.com/page/1/?s=valproic acid&search_id=product&post_type=product

-CHIR99021:
stemcell.com/products/chir99021.html
The purpose of CHIR is to inhibit GSK3, which it does on both pathways of GSK3, which are GSK3a and GSK3b. There are alternative GSK3 inhibitors you can take if you want to lower the amount of experimental drugs in this stack that have no research on humans. I'd recommend using ChatGPT to find them. If you can't find one that inhibits both pathways you could take a GSK3 inhibitor that inhibits pathway A and another that inhibits pathway B. Rosmarinic acid inhibits both GSK3 pathways
a.co/d/4Jw3qh7
It may be smart to wear a continuous glucose monitor to watch your blood sugar levels as GSK3 inhibitors drop your blood sugar which I've personally noticed when using Curcumin, which inhibits GSK3b. You can get an online prescription for a CGM easily from this website by saying you have diabetes:
tastermonial.com/products/cgm-freestyle-libre-rx

I've heard good things about the Dexcom G7 and the Freestyle Libre. I'd recommend getting a finger prick glucose meter so you can make sure the CGM is giving you the correct data.

-RepSox/E-616452:
stemcell.com/products/repsox.html

aging-us.com/article/204896/text

Future work will be directed to understanding how long the effects of these and other EPOCH treatments last and whether they reverse aspects of aging and extend lifespan in mice[7, 8, 26].

-Tranylcypromine
aipctshop.com/?s=tranylcypromine&search_id=product&post_type=product

Forskolin:
amazon.com/s?k=forskolin&crid=21VNVWZFSQZNO&sprefix=forskol,aps,721&ref=nb_sb_ss_ts-doa-p_1_7

-Sodium butyrate:
amazon.com/s?k=sodium butyrate&crid=UB47BKKGB4X8&sprefix=sodium butyrate,aps,341&ref=nb_sb_noss_1

Alternatively you can use Tributyrin as it's a more bioavailable form of sodium butyrate
amazon.com/s?k=Tributryn&crid=2CQCA050YXHO1&sprefix=tributryn ,aps,289&ref=nb_sb_noss_2

It may be smart to take this for only 4 days and then take a break for 7 to reduce the chances of things going wrong. In the study it mentioned that 4 days of this treatment resulted in the measured age being reduced by more than 3 years.

cocktail of elements

you have to blend it up with some vodka anon

Declaration of interests

D.A.S. is a consultant, inventor, board member, and in some cases a founder and investor in Life Biosciences (a reprogramming company), EdenRoc Sciences/Cantata/Dovetail/Metrobiotech, InsideTracker, Fully Aligned, Zymo, Athletic Greens, Levels Health, Galilei, Immetas, Animal Biosciences, Tally Health, and others.

Simplified Explanation:
Think of aging like a scratched DVD—over time, the information gets harder to read. Scientists found a way to "polish" the DVD using specific chemicals, restoring the original clarity without changing the content. These chemical cocktails help cells regain their youthful function, potentially leading to treatments for age-related diseases and overall rejuvenation

reversed aging

not just, stop aging

what happens if you keep taking it?

benjamin butthole

they cure cancer, alzheimers, MS and balding every single day in mice tests and have done so for the last 20 years yet we still dont have a cure to those for humans. wake me up when something actually works in humans.

I'm ok with this, should kill the mouse germs

Sounds sus and Jewish

Nice. Thanks.

Tranylcypromine

Used to treat depression, is a MAOI

Forskolin

Raises cAMP, whatever that is. Used for cellular communication

Sodium butyrate

It's a salt form of butyric acid which is found in butter.

The only one I'd be comfortable taking is the butyrate, unless there was more safety studies done.

you scratched my cd

maybe the answer is to turn humans more like rats with genesplicing
ratmen will be the next form of evolution with all diseases disposed of

Aajonus Vonderplanitz already figured out and found the cure for all these things decades ago.

They are ridiculously easy to cure and prevent. The first one, cancer, is not even a disease.

You picked it up in broad daylight and scratched it

finally, some forward thinking. fund this man.

Foreskin grows back

foreskinolin

That explains a lot.

Cancer is a metabolic issue where your cells switch from aerobic to anaerobic energy production. It could also be a symptom of parasite/fungal infection.

Cancer is cured with literally heat packs and dog dewormer. There was an Australian dr lady who cured tumors with simple hot/cold therapy to disrupt the tumors homeostasis.

In case you are wondering, their females are hideous so you wouldn't want to fuck one, my good furry.

t. has been into Warhammer for almost 20 years.

id fuck a rat lady

Have fun. If i were you though, Id put on a condom

Cancer is a metabolic issue

your body is a compendium of cells acting in concert to be you. some of those cells go rogue and revolt against the whole. we must crush these insurrectionists and

where am I going with this
nvm

Wrong. Cancer cells are basically trash collectors. They are one of the mechanisms the body has to get rid of dead cells.
They collect cells that are too toxic to dissolve and leave them all in the same place, where a tumor forms.
The difference between a "malignant" and "benign" tumor is whether the cancer cells have started to dissolve the toxic dead cells or not, respectively.
Once they do, all the toxins stored in them are released and that's why you get sick.
Cancer cells are not detrimental. They are a mechanism of your body trying to save your life. We all have cancer, and it's a good thing.

Cancer at its core is caused by either an overexposure to toxins, or a deficiency in saturated animal fat.
Your body needs saturated fat to deal with toxins, and most people are deficient in it.

i wonder if something traumatic happens in a persons life to end up this contrarian or if they just pick it up as a hobby

Not an argument.

it would be very painful

there is one fruit / nut that has tremendous amount of saturated fat

oct4, sox2, klf4

Pluripotent stem cells

Yamanaka factors

Imagine believing this crap lmao

Plants do not have fat. They have oils, which burn at the same 9kcal/gram as fat, but they are not fat.

Valproic acid

Terrible idea. This is a really nasty drug with a ton of side effects including liver damage.

there is one plant has the right type of oil and acid and it is available in your grocery store

Would be nice fr fr

Lol
Interesting theory. I don't think the body can move cells like that.

it will be damaged but younger

this is the rat cocktail. the one they made for human cells didn't work, per the article in your link. however I did notice something interesting:

A recent, unpublished study reported that 6T pre-treatment prevents senescence in human fibroblasts

that would be RepSox and Tranylcypromine, however both of them have side effects that I'd rather not test

glorious shota revival century

Give me my fucking rat husband, science

Cancer cells are not detrimental.

hu?
people literally die from it

I'm not a mouse

There is no profit in human medicine. The mice control all the gold.

Finally I can Anon Babble

cute mice

Now its time to build a nazi master race

Tranylcypromine

Forskolin

Tranny foreskin?

Cancer is parasites
Cancer is cured by antiparasitical meedications or fasting

Tl;dr

Some rich asshole is gonna monopolize this cocktail with a prize tag so vast that only the top 1% can afford it, to remain young and you'll never see it and die. Have fun

Forskolin

I wonder where that comes from

It may be smart to take this for only 4 days and then take a break for 7 to reduce the chances of things going wrong. In the study it mentioned that 4 days of this treatment resulted in the measured age being reduced by more than 3 years.

I think you need to be at least 50+ to notice rejuvenation because this is the age where you start to break down in an obvious way. If a 30 year old takes this, hes not going to notice any real difference.

increased risk of cancer

i wonder if something traumatic happens in a persons life to end up this contrarian or if they just pick it up as a hobby

I think this is a chatbot trying to impersonate a deranged human.

Buy enough supplies for 100 treatments before they manage to lock it down.

Nah, 40 year olds will also certainly notice. I'm 41 and in very good health, but I am definitely not 30 anymore.

If you just drink a handle of liquor every 2 days for about 8 years you can feel like you're 50 before you even reach 30. Don't ask me how I know

WTF these mice get to be rejuvenated while I'm losing my hair